Author:
Srikakulapu Prasad,Pattarabanjird Tanyaporn,Upadhye Aditi,Bontha Sai Vineela,Osinski Victoria,Marshall Melissa A.,Garmey James,Deroissart Justine,Prohaska Thomas A.,Witztum Joseph L.,Binder Christoph J.,Holodick Nichol E.,Rothstein Thomas L.,McNamara Coleen A.
Abstract
Immunoglobulin M (IgM) to oxidation specific epitopes (OSE) are inversely associated with atherosclerosis in mice and humans. The B-1b subtype of B-1 cells secrete IgM to OSE, and unlike B-1a cells, are capable of long-lasting IgM memory. What attributes make B-1b cells different than B-1a cells is unknown. Our objectives were to determine how B-1b cells produce more IgM compared to B-1a cells at homeostatic condition and to see the differences in the B-1a and B-1b cell distribution and IgM CDR-H3 sequences in mice with advanced atherosclerosis. Here,in-vivostudies demonstrated greater migration to spleen, splenic production of IgM and plasma IgM levels inApoE-/-Rag1-/-mice intraperitoneally injected with equal numbers of B-1b compared to B-1a cells. Bulk RNA seq analysis and flow cytometry of B-1a and B-1b cells identified CCR6 as a chemokine receptor more highly expressed on B-1b cells compared to B-1a. Knockout of CCR6 resulted in reduced B-1b cell migration to the spleen. Moreover, B-1b cell numbers were significantly higher in spleen of aged atheroscleroticApoE-/-mice compared to youngApoE-/-mice. Single cell sequencing results of IgHM in B-1a and B-1b cells from peritoneal cavity and spleen of atherosclerotic agedApoE-/-mice revealed significantly more N additions at the V-D and D-J junctions, greater diversity in V region usage and CDR-H3 sequences in B-1b compared to B-1a cells. In summary, B-1b cells demonstrated enhanced CCR6-mediated splenic migration, IgM production, and IgM repertoire diversification compared to B-1a cells. These findings suggest that potential strategies to selectively augment B-1b cell numbers and splenic trafficking could lead to increased and more diverse IgM targeting OSE to limit atherosclerosis.
Subject
Immunology,Immunology and Allergy
Cited by
1 articles.
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