Author:
Wang Xitao,Cheng Wei,Zeng Xingzhi,Dou Xiaolin,Zhou Zhongyi,Pei Qian
Abstract
ObjectiveThe poor prognosis and heterogeneity of stage III colon cancer (CC) suggest the need for more prognostic biomarkers. The tumor microenvironment (TME) plays a crucial role in tumor progression. We aimed to explore novel immune infiltration-associated molecules that serve as potential prognostic and therapeutic targets.MethodsTME immune scores were calculated using “TMEscore” algorithm. Differentially expressed genes between the high and low TME immune score groups were identified and further investigated through a protein-protein interaction network and the Molecular Complex Detection algorithm. Cox regression, meta-analysis and immunohistochemistry were applied to identify genes significantly correlated with relapse-free survival (RFS). We estimated immune infiltration using three different algorithms (TIMER 2.0, CIBERSORTx, and TIDE). Single-cell sequencing data were processed by Seurat software.ResultsPoor RFS was observed in the low TME immune score groups (log-rank P < 0.05). EPSTI1 was demonstrated to be significantly correlated with RFS (P < 0.05) in stage III CC. Meta-analysis comprising 547 patients revealed that EPSTI1 was a protective factor (HR = 0.79, 95% CI, 0.65-0. 96; P < 0.05)). More immune infiltrates were observed in the high EPSTI1 group, especially M1 macrophage and myeloid dendritic cell infiltration (P < 0.05).ConclusionThe TME immune score is positively associated with better survival outcomes. EPSTI1 could serve as a novel immune prognostic biomarker for stage III CC.
Subject
Immunology,Immunology and Allergy
Reference44 articles.
1. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries;Sung;CA: A Cancer J Clin,2021
2. TNM classification of malignant tumours;Brierley;John Wiley Sons;,2017
3. Duration of adjuvant chemotherapy for stage III colon cancer;Grothey;N Engl J Med,2018
4. Understanding the tumor immune microenvironment (TIME) for effective therapy | nature medicine
5. Cancer evolution constrained by the immune microenvironment;McGranahan;Cell,2017
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