Author:
Zhang Jiangsheng,Nan Yi,Su Jie,Jibril Aminu Usman,Lv Guiyuan
Abstract
Leptadenia hastata (Pers.) Decne is a commonly used food source and prescribed as a traditional African medicine for treatment of various diseases, such as diabetes, skin disorders, wounds, and ulcers. However, quality control has become a bottleneck restricting the therapeutic development and utilization of this plant. In this study, a reliable method for qualitative and quantitative determination of components in Leptadenia hastata was established. The components of L. hastata were profiled using ultra-high performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS). Subsequently, an ultra-high performance tandem diode array detector (UHPLC-DAD)-based method was used for simultaneous quantitative analysis of five major constituents in six batches of L. hastata samples. As a result, 35 compounds were tentatively identified. The quantities of the five constituents (vicenin-Ⅱ, orientin, schaftoside, chrysin 6-C-arabinoside 8-C-glucoside, chrysin 6-C-glucoside 8-C-arabinoside) were determined as 124.8–156.9 μg/g, 170.5–216.0 μg/g, 61.31–93.73 μg/g, 85.13–119.3 μg/g and 99.82–129.4 μg/g, respectively. This method offers a successful strategy for precise and effective evaluation of the constituents of L. hastata, providing a robust foundation for holistic quality assessment of medicinal plants.
Reference26 articles.
1. Therapeutic potential of green synthesized gold nanoparticles using extract of Leptadenia hastata against invasive pulmonary aspergillosis;Abdallah;J. Fungi,2022
2. Validating the medicinal potential of Leptadenia hastata;Aliero;Afr. J. Pharm. Pharmacol.,2009
3. New polyoxypregnane ester derivatives from Leptadenia hastata;Aquino;J. Nat. Prod.,1995
4. Anti-androgenic activity of Leptadenia hastata (Pers.) Decne: competitive effect of the aqueous extracts of the plant and the testosterone propionate on castrated immature rats;Bayala;Biotechnol. Agron. Soc. Environ.,2011
5. Assessing antimicrobial agents of Nigeria flora;Bello;J. King Saud. Univ.– Sci.,2019