Author:
Spratt Spencer J.,Mizuguchi Takaha,Akaboshi Hikaru,Kosakamoto Hina,Okada Rina,Obata Fumiaki,Ozeki Yasuyuki
Abstract
Introduction: Visualizing small individual biomolecules at subcellular resolution in live cells and tissues can provide valuable insights into metabolic activity in heterogeneous cells, but is challenging.Methods: Here, we used stimulated Raman scattering (SRS) microscopy to image deuterated methionine (d-Met) incorporated into Drosophila tissues in vivo.Results: Our results demonstrate that SRS can detect a range of previously uncharacterized cell-to-cell differences in d-Met distribution within a tissue at the subcellular level.Discussion: These results demonstrate the potential of SRS microscopy for metabolic imaging of less abundant but important amino acids such as methionine in tissue.
Funder
Japan Society for the Promotion of Science
Core Research for Evolutional Science and Technology
Ministry of Education, Culture, Sports, Science and Technology
Cited by
2 articles.
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