KDEON WK-11: A short antipseudomonal peptide with promising potential

Abstract

The plight of antimicrobial resistance continues to limit the availability of antibiotic treatment effective in combating resistant bacterial infections. Despite efforts made to rectify this issue and minimise its effects on both patients and the wider community, progress in this area remains minimal. Here, wede-novodesigned a peptide named KDEON WK-11, building on previous work establishing effective residues and structures active in distinguished antimicrobial peptides such as lactoferrin. We assessed its antimicrobial activity against an array of bacterial strains and identified its most potent effect, againstPseudomonas aeruginosawith an MIC value of 3.12 μM, lower than its counterparts developed with similar residues and chain lengths. We then determined its anti-biofilm properties, potential mechanism of action andin vitrocytotoxicity. We identified that KDEON WK-11 had a broad range of antimicrobial activity and specific capabilities to fightPseudomonas aeruginosawith lowin vitrocytotoxicity and promising potential to express anti-lipopolysaccharide qualities, which could be exploited to expand its properties into an anti-sepsis agent.