Author:
Potowski Marco,Kunig Verena B. K.,Eberlein Lukas,Škopić Mateja Klika,Vakalopoulos Alexandros,Kast Stefan M.,Brunschweiger Andreas
Abstract
DNA-encoded libraries are a prime technology for target-based small molecule screening. Native DNA used as genetic compound barcode is chemically vulnerable under many reaction conditions. DNA barcodes that are composed of pyrimidine nucleobases, 7-deazaadenine, and 7-deaza-8-azaguanine have been investigated for their suitability for encoded chemistry both experimentally and computationally. These four-letter barcodes were readily ligated by T4 ligation, amplifiable by Taq polymerase, and the resultant amplicons were correctly sequenced. Chemical stability profiling showed a superior chemical stability compared to native DNA, though higher susceptibility to depurination than a three-letter code based on pyrimidine DNA and 7-deazaadenine.
Funder
Bayer
Deutsche Forschungsgemeinschaft
Cited by
1 articles.
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