Author:
Yin Qikai,Cheng Rui,Xu Xiuyan,Xu Ziqian,Wang Jing,Fu Shihong,Xu Hongbin,Zhang Shaozai,He Ying,Li Fan,Xu Songtao,Lu Xiaoqing,Wang Huanyu,Wang Bin,Liang Guodong
Abstract
In July 2018, a virus (JXLC1806-2) was isolated from Culicoides biting midges collected in Lichuan County, Jiangxi Province, China. The virus isolate showed significant cytopathic effects within 48 hours after inoculation with mammalian cells (BHK-21). JXLC1806-2 virus could form plaques in BHK-21 cells, and the virus titer was 1×105.6 pfu/mL. After inoculation with the virus, suckling mice developed disease and died. The nucleotide and amino sequence analysis showed that the JXLC1806-2 virus genome was composed of S, M and L segments. Phylogenetic analysis showed that the S, M and L genes of JXLC1806-2 virus belonged to the Tete serogroup, Orthobunyavirus, but formed an independent evolutionary branch from the other members of the Tete serogroup. The results showed that the JXLC1806-2 virus, which was named as Lichuan virus, is a new member of Tete serogroup, and this is the first time that a Tete serogroup virus has been isolated in China.
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology