Author:
da Silva Elenice Francisco,Bastos Luciana Machado,Fonseca Belchiolina Beatriz,Ribas Rosineide Marques,Sommerfeld Simone,Pires Henrique Machado,dos Santos Fernanda Aparecida Longato,Ribeiro Lígia Nunes de Morais
Abstract
Lately, the bacterial multidrug resistance has been a reason to public health concerning around world. The development of new pharmacology therapies against infections caused by multidrug-resistant bacteria is urgent. In this work, we developed 10 NLC formulations composed of essential oils (EO), vegetable butter and surfactant. The formulations were evaluated for long-term and thermal cycling stability studies in terms of (particle size, polydispersion index and Zeta potential). In vitro antimicrobial assays were performed using disk diffusion test and by the determination of the minimum inhibitory concentration (MIC) performed with fresh and a year-old NLC. The most promising system and its excipients were structurally characterized through experimental methodologies (FTIR-ATR, DSC and FE-SEM). Finally, this same formulation was studied through nanotoxicity assays on the chicken embryo model, analyzing different parameters, as viability and weight changes of embryos and annexes. All the developed formulations presented long-term physicochemical and thermal stability. The formulation based on cinnamon EO presented in vitro activity against strains of Acinetobacter baumannii, Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from humans and in vivo biocompatibility. Considering these promising results, such system is able to be further tested on in vivo efficacy assays.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Reference46 articles.
1. AmBisome: liposomal formulation, structure, mechanism of action and pre-clinical experience;Adler-Moore;J. Antimicrobial Chemother.,2002
2. Lipid-based carriers for the delivery of local anesthetics;Araújo;Expert Opin. Drug Delivery,2019
3. Resistance-resistant antibacterial treatment strategies;Batchelder;Front. Antibiotics,2023
4. Multi-hospital point prevalence study of healthcare-associated infections in 28 adult intensive care units in Brazil;Braga;J. Hosp. Infection,2018
5. Mucoadhesive, thermoreversible hydrogel, containing tetracaine-loaded nanostructured lipid carriers for topical, intranasal needle-free anesthesia;Calixto;Pharmaceutics,2021
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献