Author:
Guo Yiwen,Lin Yun,Xie Quan,Zhang Wenyuan,Xu Zhenqi,Chao Yifei,Cao Xudong,Jiang Huiru,Li Han,Li Tuofan,Wan Zhimin,Shao Hongxia,Qin Aijian,Ye Jianqiang
Abstract
Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.
Funder
National Key Research and Development Program of China
Basic Research Program of Jiangsu Province
National Natural Science Foundation of China
China Postdoctoral Science Foundation
Priority Academic Program Development of Jiangsu Higher Education Institutions
Subject
Infectious Diseases,Microbiology (medical),Immunology,Microbiology
Cited by
2 articles.
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