Author:
Cortes Garcia Esteban,Giarraputo Alessia,Racapé Maud,Goutaudier Valentin,Ursule-Dufait Cindy,de la Grange Pierre,Adoux Lucie,Raynaud Marc,Couderau Clément,Mezine Fariza,Dagobert Jessie,Bestard Oriol,Moreso Francesc,Villard Jean,Halleck Fabian,Giral Magali,Brouard Sophie,Danger Richard,Gourraud Pierre-Antoine,Rabant Marion,Couzi Lionel,Le Quintrec Moglie,Kamar Nassim,Morelon Emmanuel,Vrtovsnik François,Taupin Jean-Luc,Snanoudj Renaud,Legendre Christophe,Anglicheau Dany,Budde Klemens,Lefaucheur Carmen,Loupy Alexandre,Aubert Olivier
Abstract
Recently, interest in transcriptomic assessment of kidney biopsies has been growing. This study investigates the use of NGS to identify gene expression changes and analyse the pathways involved in rejection. An Illumina bulk RNA sequencing on the polyadenylated RNA of 770 kidney biopsies was conducted. Differentially-expressed genes (DEGs) were determined for AMR and TCMR using DESeq2. Genes were segregated according to their previous descriptions in known panels (microarray or the Banff Human Organ Transplant (B-HOT) panel) to obtain NGS-specific genes. Pathway enrichment analysis was performed using the Reactome and Kyoto Encyclopaedia of Genes and Genomes (KEGG) public repositories. The differential gene expression using NGS analysis identified 6,141 and 8,478 transcripts associated with AMR and TCMR. While most of the genes identified were included in the microarray and the B-HOT panels, NGS analysis identified 603 (9.8%) and 1,186 (14%) new specific genes. Pathways analysis showed that the B-HOT panel was associated with the main immunological processes involved during AMR and TCMR. The microarrays specifically integrated metabolic functions and cell cycle progression processes. Novel NGS-specific based transcripts associated with AMR and TCMR were discovered, which might represent a novel source of targets for drug designing and repurposing.