Author:
Charles Ciriac,Conde Cyril,Biet Franck,Boschiroli Maria Laura,Michelet Lorraine
Abstract
IS6110 is an insertion sequence found in the Mycobacterium tuberculosis complex, to which Mycobacterium bovis belongs, which can play a role in genome plasticity and in bacterial evolution. In this study, the abundance and location of IS6110 on M. bovis genomic data of French animal field strains were studied. A first analysis was performed on a panel of 81 strains that reflect the national M. bovis population’s genetic diversity. The results show that more than one-third of them are IS6110 multicopy and that 10% have IS6110 in a high copy number (more than 6 copies). Multicopy strains are those circulating in the regions where prevalence was above the national average. Further study of 93 such strains, with an IS6110 copy number of 10-12, showed stability of IS6110 copy number and genome location over time and between host species. The correlation between M. bovis multicopy strains and high bovine tuberculosis (bTB) prevalence leads us to consider whether their epidemiological success could be partly due to genetic changes originated by IS6110 transposition.
Funder
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
Subject
Microbiology (medical),Microbiology
Reference60 articles.
1. Evaluation of the Epidemiological Relevance of Variable-Number Tandem-Repeat Genotyping of Mycobacterium bovis and Comparison of the Method with IS6110 Restriction Fragment Length Polymorphism Analysis and Spoligotyping.;Allix;J. Clin. Microbiol.,2006
2. Mapping IS6110 in high-copy number Mycobacterium tuberculosis strains shows specific insertion points in the Beijing genotype.;Alonso;BMC Genomics,2013
3. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing.;Bankevich;J. Comput. Biol.,2012
4. Lutte contre la tuberculose bovine en France de 1954 à 2004: Analyse de la pertinence épidémiologique de l’évolution de la réglementation.;Benet;Epidémiol et santé anim.,2006
5. Tuberculose bovine en France : cartographie des souches de Mycobacterium bovis entre 2000-2013.;Boschiroli;Bull. Épidémiol. santé animale alimentation.,2015
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