Microbial Succession and Interactions During the Manufacture of Fu Brick Tea

Author:

Xiang Meichun,Chu Jun,Cai Wenjiao,Ma Haikun,Zhu Weijing,Zhang Xiaoling,Ren Jinwei,Xiao Lizheng,Liu Dongbo,Liu Xingzhong

Abstract

Fu Brick tea is a very popular post-fermented tea that is known for its “golden flower fungus,” Aspergillus cristatus, which becomes the dominant microbe during the maturation process. This study used both culture-dependent methods and high-throughput sequencing to track microbial succession and interactions during the development of the golden flower fungus, a crucial component of the manufacturing process of Fu Brick tea. Among the bacterial communities, Klebsiella and Lactobacillus were consistently cultured from both fresh tea leaves and in post-fermentation Fu Brick tea. Methylobacterium, Pelomonas, and Sphingomonas were dominant genera in fresh tea leaves but declined once fermentation started, while Bacillus, Kluyvera, and Paenibacillus became dominant after piling fermentation. The abundance of A. cristatus increased during the manufacturing process, accounting for over 98% of all fungi present after the golden flower bloom in the Fu Brick tea product. Despite their consistent presence during culture work, network analysis showed Lactobacillus and Klebsiella to be negatively correlated with A. cristatus. Bacillus spp., as expected from culture work, positively correlated with the presence of golden flower fungus. This study provides complete insights about the succession of microbial communities and highlights the importance of co-occurrence microbes with A. cristatus during the manufacturing process of Fu Brick tea.

Publisher

Frontiers Media SA

Subject

Microbiology (medical),Microbiology

Reference65 articles.

1. The UNITE database for molecular identification of fungi–recent updates and future perspectives;Abarenkov;N. Phytol.,2012

2. Improvement of tea leaves fermentation with Aspergillus spp. pectinase;Angayarkanni;J. Biosci. Bioeng.,2002

3. GenBank;Benson;Nucleic Acids Res.,2005

4. Molecular systematics of some yeast-like anamorphs belonging to the Ustilaginales and Tilletiales;Boekhout;Stud. Mycol.,1995

5. Production of poly-β-hydroxybutyrate from methanol: characterization of a new isolate of Methylobacterium extorquens;Bourque;Appl. Microbiol. Biotechnol.,1992

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3