Hydrolytic Enzyme Activities and Siderophore Production Capabilities of Pathogenic Bacterial Isolates from Rainbow Trout

Abstract

The fact that they can be cultured in large quantities in a short time, that they are open to genetic manipulations, and that the enzymes they produce are more active and stable than animal and plant enzymes, cause bacteria to be considered as very important enzyme and secondary metabolite sources. Also, extracellular hydrolytic enzymes and siderophores are known as very important virulence factors for pathogenic bacteria. In this study, the ability to produce various extracellular hydrolytic enzymes and siderophores of nine bacterial isolates isolated from rainbow trout from farms operating in Van province was investigated for the first time. The results showed that of the tested bacteria, nine had protease, eight had lipase, five had cellulase, two had pectinase activity, and seven had the ability to produce siderophores. Amylase and xylanase activities were not observed in any isolate. In addition, these isolates were morphologically divided into three possible main groups and three isolates from each group were molecularly identified with primers developed specifically for the species. Molecular identification using species-specific primers revealed that these isolates could be the strains of Listonella anguillarum, Yersinia ruckeri and Lactococcus garvieae.