Antioxidant, iron chelating, lipase inhibition activities and metabolite’s prediction of hyrdoethanolic leaf extract of Conocarpus erectus

Abstract

The current study was conducted to evaluate the antioxidant, Fe-chelating and lipase inhibition activities of hydroethanolic leaf extracts of Conocarpus erectus. The proton magnetic resonance spectroscopy (1H-NMR) was used to identify the major types of primary and secondary metabolites in extract. The 60% ethanolic extract was found to be the most effective fraction regarding antioxidant, Fe chelating and lipase inhibitory properties. The 60% ethanolic leaf extract exhibited total antioxidant power of 223.0±3.27 mg ASE/g PE, β-carotene bleaching inhibition of 81.39±2.11%, Fe-chelating of 68.21±1.17% and pancreatic lipase inhibition of 50.60±1.47%, respectively. The 1H-NMR -based prediction of metabolites provided the information for presence of polyphenolic secondary metabolites and organic acids which might be responsible for the biological activities of extract. These findings of this work may be extended for metabolite characterization, in vivo trials and toxicity determination to get benefits for functional food development with antioxidative and antiobesity attributes.