Affiliation:
1. Oles Honchar Dnipro National University
2. Kerch State Maritime Technological University
Abstract
Imidacloprid is a widely used pesticide that belongs to the class of neonicotinoids. There is a piece of rising evidence that neonicotinoids exert cytotoxic effects in non-target organisms including vertebrate species such as mammals. Nevertheless, dose-limiting toxicity and molecular mechanisms of neonicotinoids' deleterious effects are still poorly understood. In accord to imidacloprid fate in the environment, the most of used pesticide is absorbed in the soil. Therefore, earthworms, which are prevailing soil organisms, could be considered as a target of neonicotinoids toxicity. The earthworm’s simple nervous system is a prospective model for neurotoxicological studies. We exposed earthworms to imidacloprid in a paper contact test with a doses range of 0.1‑0.4 µg/cm2 for 14 days. In the present work, we studied the imidacloprid effect on oxidative stress generation and neuronal marker neuron-specific enolase (NSE) expression. The exposure to imidacloprid induced a dose-dependent decrease in NSE. Both reactive oxygen species production and lipid peroxidation level were upregulated as well. Observed NSE decline suggests imidacloprid-caused disturbance in earthworm neuron cells. Obtained data have shown that relatively low doses of imidacloprid are potent to induce cytotoxicity in neurons. Furthermore, neurotoxicity could be recognized as one of an individual scenario of the general imidacloprid toxicity. Thus, presented results suggest the cytotoxicity of imidacloprid low doses in non-target organisms and hypothesize that NSE downregulation could be estimated as a biomarker of neonicotinoid cytotoxicity in a nervous system of non-insect species.
Reference46 articles.
1. E.E. Ewere et al., Uptake, depuration and sublethal effects of the neonicotinoid, imidacloprid, exposure in Sydney rock oysters, Chemosphere. 230 (2019) 1–13.
2. E.A.D. Mitchell et al., A worldwide survey of neonicotinoids in honey, Science (New York, N.Y.). 358(6359) (2017) 109–111.
3. Ph. Wexler (Ed.) Encyclopedia of Toxicology, Academic Press, Elsevier Inc., (2014).
4. I.Y. Abd-Elhakim et al., Imidacloprid impacts on neurobehavioral performance, oxidative stress, and apoptotic events in the brain of adolescent and adult rats, Journal of Agricultural and Food Chemistry. 66(51) (2018) 13513–13524.
5. L. Donnarumma et al., Preliminary study on persistence in soil and residues in maize of imidacloprid, Journal of Environmental Science and Health, Part B. 46(6) (2011) 469–472.
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