Author:
Saveleva Anna E.,Kurkina Anna V.
Abstract
The spreading marigold (Tagetes patula L.) is a prospective source of flavonoids. The aim of the study is to develop methods of standardization of spreading marigold flowers, consisting in determining the identity of this raw material and the quantitative determination of biologically active compounds. As a result of a comparative chromatographic study, the presence of flavonoids was revealed at the wavelength of 254 and 366 nm before and after the reaction with the alcohol solution of aluminum chloride. The method of determination of patulitrin and patuletin in the spreading marigold flowers (Tagetes patula L.) with the use of high performance liquid chromatography was developed. The content of dominant flavonoid patulitrin in the flowers of T. patula varies from 5.11 0.18% to 5.64 0.17%. The error of single determination of patulitrin in the spreading marigold flowers with confidence probability of 95% is 3.32%.
Publisher
Samara State Medical University
Reference6 articles.
1. Zaitceva EN, Dubishchev AV, Kurkin VA. Analysis of influence of rutin and gravity action on the renal excretory function. Nauka i innovacii v medicine. 2016;(4):47–50. (In Russ.)
2. Kurkina AV. Flavonoidy farmakopejnyh rastenij: monografiya. Samara; 2012. (In Russ.)
3. Lomkina EM, Chervonnaya NM, Oganesyan ET, et al. Tyurenkov effect of french marigold (Tagetes patula L.) extract on wound healing in diabetes mellitus. Farmatsiia. 2016;65(3):37–39. (In Russ.)
4. ON THE CONTENT OF PHENOLIC COMPOUNDS IN THE CONCENTRATIONS OF THE TAGETES PATULA L.