An approach to standardization of licorice herb (<i>glycyrrhiza glabra</i> l.)-Reference-Cited by-同舟云学术

An approach to standardization of licorice herb (<i>glycyrrhiza glabra</i> l.)

Published:2023-06-16 Issue:2 Volume:23 Page:53-59
ISSN:2410-3764
Container-title:Aspirantskiy Vestnik Povolzhiya
language:
Short-container-title:Aspirantskiy Vestnik Povolzhiya

Author:

Belova Olga A.^ORCID,Kurkin Vladimir A.^ORCID,Smirnova Elizaveta A.^ORCID,Egorov Maksim V.^ORCID

Abstract

Aim to develop a method for standardization of licorice herb. Material and methods. The material for the study was licorice herb harvested in Alekseevka settlement of the Samara Region (2021), Tatarskaya Kargala village of Sakmarskiy area of the Orenburg Region (2017), Derzhavinsk city of the Republic of Kazakhstan (2018), Bol'shaya Chernigovka village of Bol'shchernigovsk area of the Samara Region (2019), and a commercial sample of licorice root raw material from AO "Krasnogorskleksredstva". In addition, the reference standards (RS) were used: pinostrobin, licuraside, quercetin, rutin, luteolin and glycyram. The authenticity was determined by the thin layer chromatography (qualitative analysis). For the quantitative analysis, we used the differential spectrophotometry, conducted in accordance with Pharmacopoeial monograph 1.2.1.1.0003.15 "Spectrophotometry in ultraviolet and visible spectra". The spectral characteristics of water-alcohol extracts were evaluated on spectrophotometer "Specord 40" (Analytik Jena AG, Germany). Results. The dominant flavonoid substance, pinocembrin, was detected. This compound in chloroform-ethanol (4:1) solvent system had blue fluorescence at 365 nm. During the development of the chromatogram with an alkaline solution of diazobenzene sulphonic acid, a light green stain (pinoсembrin) was detected practically at the level of the pinostrobin stain. It is reasonable to determine the diagnostic flavonoid pinocembrin of licorice herb using pinostrobin as the reference standard. The flavonoids pinocembrin and pinostrobin have similar spectral characteristics and are similar in chemical structure. A method has been developed for quantitative determination of the total flavonoids in terms of pinocembrin in water-ethanol extract of licorice herb (Glycyrrhiza glabra L.) using the differential spectrophotometry. The total flavonoid content in the licorice herb varies from 0.390.002% to 3.480.015% (in terms of pinocembrin).

Publisher

Samara State Medical University

Link

https://aspvestnik.ru/2410-3764/article/viewFile/112144/pdf

Reference21 articles.

1. Aseeva TA, Blinova KF, Yakovlev GP. Medicinal developments of Tibetan medicine. Novosibirsk, 1985. (In Russ.). [Асеева Т.А., Блинова К.Ф., Яковлев Г.П. Лекарственные растения тибетской медицины. Новосибирск, 1985].

2. Kruganova EA. A review of the species Glycyrrhiza L. and Meristotropis Fisch. et Mey. In: Proceedings of the Botanical Institute of the Academy of Sciences of the USSR. 1955;1(2):161-197. (In Russ.). [Круганова Е.А. Обзор видов Glycyrrhiza L. и Meristotropis Fisch. et Mey. В кн.: Труды Ботанического института АН СССР. 1955;1(2):161-197].

3. Chikov PS. Atlas of Areals and Resources of Medicinal Plants of the USSR. M., 1980. (In Russ.). [Чиков П.С. Атлас ареалов и ресурсов лекарственных растений СССР. М., 1980].

4. Grigor'ev YuS, Vasil'chenko IT. Flora of the USSR. Vol. 13, M., L., 1948:230-239. (In Russ.). [Григорьев Ю.С., Васильченко И.Т. Флора СССР. Т. 13, М., Л., 1948:230-239].

5. The State Pharmacopoeia оf the Russian Federation, XIV ed. [Electronic resource]. (In Russ.). [Государственная фармакопея Российской Федерации XIV изд. (электронное издание)]. Available at: https://femb.ru/record/pharmacopea14

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