COMPARISON OF N-ACETYLENE CYSTEINE AND RESVERATROL TREATMENTS IN ACETAMINOPHEN TOXICITY MODEL IN RABBITS

Abstract

Purpose: Acetaminophen is safe drug but it may cause hepatic failure in high doses. N-acetyl cysteine (NAC) is used in treatment of acetaminophen intoxication. Resveratrol may be used in experimental acetaminophen intoxication models. Purpose, compare the efficacy of NAC and resveratrol treatments in acetaminophen intoxication. Materials and Methods: We used 34 rabbits. 6 rabbits were included in control group and 7 rabbits were enrolled in other groups APAP group, animals administered 2 g/kg of acetaminophen by orogastric tube. APAP + NAC group, 150 mg/kg NAC was administered intramusculer (i.m) to animals at 1st hour following 2 g/kg of acetaminophen administration by orogastric tube. APAP + RES group, animals received 100 mg/kg resveratrol by orogastric tube at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. APAP + NAC + RES group, animals received 100 mg/kg resveratrol by orogastric tube and 150 mg/kg NAC i.m at 1st hour following oral administration of 2 g/kg of acetaminophen by orogastric tube. ALT, AST, GSH and acetaminophen levels were measured. Animals were sacrificed at 24th hour Liver samples were obtained for histopathological examination. Results: According to our results, GSH level at 12th hour was significantly different between APAP + NAC group and APAP + RES group (p = 0.007). In histopathological examination, HSCORE level we used for apoptosis was statistically significantly lower in control group than in APAP group (p = 0.007). Its supports that acetaminophen toxicity triggers apoptosis in liver cells. Conclusion: Our results support that resveratrol is option for acetaminophen toxicity.