INVESTIGATION OF THE POSSIBLE ROLE OF MACROPHAGE MIGRATION INHIBITOR FACTOR (MIF) GENE -173G/C POLYMOPHISM IN ATHEROSCLEROSIS

Abstract

Aim: Atherosclerosis is defined as an inflammatory disease that results in the formation of atherosclerotic plaques caused by the deposition of cholesterol in the arterial intima. As a result of damage to the intima layer of the vessel, foam cell formation following cholesterol accumulation and plaque development due to smooth muscle cell increase are observed. In different stages of atherosclerosis, migration of leukocytes to the vessel wall is provided by functional type chemokines; It was aimed to investigate the possible role of MIF -173G/C polymorphism in atherosclerosis disease depending on this function, since it has the same functional properties as chemokines. Material and Method: Thirty patients with 70% occlusion detected by angiography and 30 healthy individuals were included in the study. DNA isolation was performed from blood samples taken from individuals in EDTA tubes. Analysis of MIF -173G/C polymorphism was performed on Real Time PCR (LC480, Roche). Statistical analyzes were performed using the STATISTICA version 13.5.0.17 (TIBCO Software Inc. (2017)) program. Results: In the MIF -173G/C polymorphism, the frequency of GG, GC and CC genotypes in the patient group was 55.26%, 41.18% and 66.66%, and 44.74%, 58.82% and 33.33% in the control group, respectively. Compared with the GG genotype, it was determined that those with the GC genotype had a 0.567-fold (p=0.3367) risk of developing the disease, and those with the CC genotype had a 1.6190-fold (p= 0.7038) risk of developing the disease. Conclusion: Further studies can be carried out by increasing the number of samples.