Affiliation:
1. ISTANBUL UNIVERSITY, İSTANBUL FACULTY OF MEDICINE, DEPARTMENT OF INTERNAL MEDICINE, DEPARTMENT OF MEDICAL GENETICS
Abstract
Purpose: Infertility affects nearly 12% of couples worldwide, with a male factor being the primary or contributory reason in around 50% of cases. MiRNAs are essential post-transcriptional regulators in the spermatogenesis process, and dysregulated miRNAs have been shown to have harmful effects on male fertility. However, it is unclear which miRNAs are associated with infertility-related genes. The aim of this study is, to utilize various bioinformatics approaches, to identify miRNAs that may have a role in the regulation of infertility-related genes.
Materials and Methods: The study first selected genes associated with infertility from the Male Infertility Knowledge Base (MIK) database. Pathway analysis of the defined genes, PPI interaction, and hub proteins related to these genes were revealed by the Elsevier pathway collection database and Enrichr tool. Following that, miRNAs that can influence infertility-related genes were determined, and the influence of the miRNA-target gene connection on male infertility was established bioinformatically using various in silico tools like miRPathDB 2.0 tool, StarmiR, and miRNet.
Results: Male infertility associated 21genes were selected from the MIK database and 15 miRNAs were identified that are most likely to regulate these genes bioinformatically. 10 hub proteins related to defined male infertility genes were analyzed.
Conclusion: Our bioinformatic study results indicate that miR-34a-5p dysregulation may contribute to infertility through CREM, LAMP3, AGBL5, FOXM1 genes and and also miR-335-5p may cause infertility via the CFAP65, CFTR, and GAPDHS genes.
Funder
Herhangi bir kurumdan destek alınmamıştır
Publisher
Ahi Evran Medical Journal, Kirsehir Ahi Evran University
Subject
General Earth and Planetary Sciences,General Engineering,General Environmental Science
Cited by
1 articles.
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