Human Umbilical Cord Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Reduce Skin Inflammation In Vitro

Author:

Lin Tzou-Yien1,Chang Tsong-Min2ORCID,Tsai Wei-Cheng3,Hsieh Yi-Ju3,Wang Li-Ting3,Huang Huey-Chun4ORCID

Affiliation:

1. Department of Paediatrics, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 33305, Taiwan

2. Department of Hair Styling and Design, Department of Applied Cosmetology, Hungkuang University, Taichung 433304, Taiwan

3. ExoOne Bio Co., Ltd., Taipei City 115011, Taiwan

4. Department of Medical Laboratory Science and Biotechnology, College of Medicine, China Medical University, Taichung 404328, Taiwan

Abstract

The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The pretreated UVMSC-EVs showed protective activity, elevating cell viability, reducing intracellular ROS, and reducing the changes in the mitochondrial membrane potential compared to the cells with a direct oxazolone treatment alone. The UCMSC-EVs exhibited anti-inflammatory activity via reducing the inflammatory cytokines IL-1β and TNF-α. A mechanism study showed that the UCMSC-EVs increased the protein expression levels of SIRT1 and P53 and reduced P65 protein expression. It was concluded that UVMSC-EVs can induce the antioxidant defense systems of HaCaT cells and that they may have potential as functional ingredients in anti-aging cosmetics for skin care.

Funder

Ministry of Science and Technology Council

China Medical University

Industry-Academic Research Project from Hungkuang University

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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