Intracellular Sugar Transporters Facilitate Cellulase Synthesis in Trichoderma reesei Using Lactose

Author:

Wang Haiyan12,Pang Ai-Ping2,Li Bingzhi3,Huo Liujie4ORCID,Wu Fu-Gen2ORCID,Lin Fengming12ORCID

Affiliation:

1. State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300453, China

2. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

3. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

4. Helmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China

Abstract

Sugar transporters play an important role in the cellulase production of lignocellulose-degrading fungi. Nevertheless, the role and function of these transporters are still unclear. Here we first report intracellular sugar transporters assisting cellulase production in Trichoderma reesei (T. reesei) using lactose. The mRNA levels of sugar transporter genes mfs, gst, and lac1 were substantially upregulated in T. reesei cultivated on lactose, with the most abundant mRNA levels at 24 h as compared to glucose. Moreover, the individual deletion of these sugar transporters significantly inhibited cellulase production, solid cell growth, and sporulation of T. reesei, suggesting they play a supporting role in cellulase production when grown in lactose. Surprisingly, MFS, GST, and LAC1 were mainly localized in the cytoplasm, with MFS and LAC1 in the endoplasmic reticulum (ER), representing the first discovery of intracellular sugar transporters involved in cellulase biosynthesis in lactose culture. The absence of the gene lac1 noticeably inhibited most of the crucial genes related to cellulase production, including cellulase-encoding genes, transcription factors, and sugar transporters, at 24 h, which was fully relieved at 48 h or 72 h, indicating that lac1 affects cellulase production more at the early step. This research advances the understanding of the function of intracellular sugar transporters in fungi, particularly for fungal cellulase production.

Funder

National Natural Science Foundation of China

State Key Laboratory of Microbial Technology Open Project Fund

Food Nutrition and Safety

Open Project Program of State Key Laboratory of Food Nutrition and Safety

Publisher

MDPI AG

Subject

Molecular Biology,Biochemistry

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