Heterotic Effect of Different Cytoplasmic Combinations in Sunflower Hybrids Cultivated Under Diverse Irrigation Regimes

Abstract

The sunflower hybrids hold a narrow cytoplasmic diversity. Besides, the heterotic effect of wild cytoplasmic combinations of sunflower on important traits under water stress has not been explored in detail. Here, we evaluated the different sunflower cytoplasmic combinations in sunflower hybrids using cytoplasmic male sterile (CMS) sources as female parents. We used a total of sixteen sunflower genotypes representing twelve CMS lines from wild and conventional sources along with four restorer lines. Twelve CMS lines were crossed with four restorer lines to develop a total of 48 F1 hybrid combinations. The hybrids were evaluated under two different environments (i.e., regular irrigation and water stress) for morphophysiological, yield, and biochemical traits over two years. Heterotic effect for various CMS sources was evaluated on all of the three possible scales, namely, better-parent heterosis (BPH), mid-parent heterosis (MPH), and heterosis as percent of check (PSH-996). For better-parent and mid-parent heterosis, the CMS sources Helianthus annuus, Helianthus argophyllus, and Helianthus debilis demonstrated positive better-parent heterosis for seed yield, oil content, and oleic acid irrespective of the environment. However, the hybrid combinations of different sources when using the genotype RCR8297 as the restorer parent recorded maximum average returns. Furthermore, chlorophyll meter (SPAD) reading positively correlated with days to 50% flowering, days to maturity, plant height, and number of leaves per plant in both the environments. Overall, this study identified and compared the heterotic effect of the different cytoplasmic combinations in sunflower under water stress as well as under normal irrigation environments.