Effects of Gibberellin (GA4+7) in Grain Filling, Hormonal Behavior, and Antioxidants in High-Density Maize (Zea mays L.)

Abstract

Dense plant cultivation is an efficient approach to improve maize production by maximizing the utilization of energy and nutrients. However, dense plant populations may aggravate the abortion rate of young grains, resulting in fewer kernels per ear. The rate and duration of grain-filling play decisive roles in maize grain yield. Therefore, to increase plant density, enhancing the grain-filling rate, extending the growth period of individual maize plants and regulating crop senescence would be the first priority. In this study, we examined the regulatory effects of GA4+7 under two application methods: shanks and silks were moistened by cotton full with GA4+7 solution at concentrations of 0, 10, 60, and 120 mg L−1. The results showed that GA4+7 improved the grain-filling rate by increasing the content of auxin, gibberellin, zeatin, and abscisic acid in grains compared to control plants. In addition, the auxin, gibberellin, and zeatin contents in the grains were positively and significantly correlated with the maximum grain weight and the maximum and mean grain-filling rates. Moreover, GA4+7 increased the activities of superoxide dismutases, catalases, and peroxidases and reduced the malondialdehyde content in leaves compared with untreated plants. At the concentration of 60 mg L−1, GA4+7 showed the greatest effect on shank and silk applications (Sh-60 and Si-60) followed by 10 mg L−1 (Sh-10) for shank treatment and 120 mg L−1 (Si-120) for silk treatment. Our results suggest that a concentration of 60 mg L−1 GA4+7 for shank and silk application may be efficiently used for changing the level of hormones in grains and antioxidant enzymes in ear leaves, which may be useful for enhancing grain-filling rate and delaying leaf senescence, resulting in an increase in maize grain yield.