Chemical and Pharmacological Screening of Rhinella icterica (Spix 1824) Toad Parotoid Secretion in Avian Preparations

Author:

Oliveira Raquel Soares,Borges Bruna TrindadeORCID,Leal Allan PintoORCID,Lailowski Manuela Merlin,Bordon Karla de Castro FigueiredoORCID,Souza Velci Queiróz de,Vinadé LúciaORCID,Santos Tiago Gomes dosORCID,Hyslop Stephen,Moura Sidnei,Arantes Eliane CandianiORCID,Corrado Alexandre Pinto,Dal Belo Cháriston A.ORCID

Abstract

The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin B, 11α,19-dihydroxy-telocinobufagin, bufotalin, monohydroxylbufotalin, 19-oxo-cinobufagin, 3α,12β,25,26-tetrahydroxy-7-oxo-5β-cholestane-26-O-sulfate, and cinobufagin-3-hemisuberate that were identified as alkaloid and steroid compounds, in addition to marinoic acid and N-methyl-5-hydroxy-tryptamine. In chick brain slices, all fractions caused a slight decrease in cell viability, as also seen with the highest concentration of RIPS tested. In chick biventer cervicis neuromuscular preparations, RIPS and all four fractions significantly inhibited junctional acetylcholinesterase (AChE) activity. In this preparation, only fraction RI23 completely mimicked the pharmacological profile of RIPS, which included a transient facilitation in the amplitude of muscle twitches followed by progressive and complete neuromuscular blockade. Mass spectrometric analysis showed that RI23 consisted predominantly of bufogenins, a class of steroidal compounds known for their cardiotonic activity mediated by a digoxin- or ouabain-like action and the blockade of voltage-dependent L-type calcium channels. These findings indicate that the pharmacological activities of RI23 (and RIPS) are probably mediated by: (1) inhibition of AChE activity that increases the junctional content of Ach; (2) inhibition of neuronal Na+/K+-ATPase, leading to facilitation followed by neuromuscular blockade; and (3) blockade of voltage-dependent Ca2+ channels, leading to stabilization of the motor endplate membrane.

Publisher

MDPI AG

Subject

Health, Toxicology and Mutagenesis,Toxicology

Reference92 articles.

1. Amphibian Species of the World: An Online Reference;Frost,2019

2. Lista das Espécies da Fauna Ameaçadas de Extinção no Rio Grande do Sul. Decreto n. 41.672, de 11 junho de 2002, FZB/MCT–PUCRS/PANGEA, Porto Alegre, 00155http://www.tecniflora.com.br/fauna_ameacada_RS.pdf

3. Herpetologia Brasileira;Segalla,2019

4. Toxic activity and protein identification from the parotoid gland secretion of the common toad Bufo bufo

5. The Development of Toad Toxins as Potential Therapeutic Agents

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