Assessing Glycosphingolipid Profiles in Human Health and Disease Using Non-Imaging MALDI Mass Spectrometry

Author:

Novaconi Cristina1,Onulov Robert1,Serb Alina2ORCID,Sisu Eugen2,Dinca Nicolae3,Pascariu Mihai-Cosmin45,Georgescu Marius67ORCID

Affiliation:

1. Faculty of Medicine, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania

2. Biochemistry Discipline, Biochemistry and Pharmacology Department, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania

3. Department of Technical and Natural Sciences, Faculty of Food Engineering, Tourism and Environmental Protection, “Aurel Vlaicu” University, 310330 Arad, Romania

4. Department of Pharmaceutical Sciences, Faculty of Pharmacy, “Vasile Goldiș” Western University, 310414 Arad, Romania

5. National Institute of Research & Development for Electrochemistry and Condensed Matter, 300569 Timisoara, Romania

6. Physiology Discipline, Functional Sciences Department, Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania

7. Center of Immuno-Physiology and Biotechnologies (CIFBIOTEH), Victor Babes University of Medicine and Pharmacy Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania

Abstract

Glycosphingolipids (GSLs) are a glycolipid subtype which plays vital roles in numerous biological processes, cell–cell interactions, as well as oncogenesis and ontogenesis. They are ubiquitous molecules found mostly in cell membranes. Abnormal expression of GSLs as well as altered molecular structure have been linked with progression of cancer and metastasis and are involved in the pathophysiology of neurodegenerative, autoimmune, and infectious diseases as well as inherited enzyme defects—glycosphingolipidoses. Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) plays a leading role in analyzing and characterizing different GSLs, and thus can help to distinguish altered GSL patterns. This review offers insights into the benefits and limitations when using MALDI MS in this field of lipidomic research, with an emphasis on which are the optimal matrices in analyzing GSLs from different tissues (normal and pathological) as well as highlighting GSLs’ particular profiles in various cell cultures, and normal and pathological human tissues obtained by MALDI non-imaging MS (non-IMS). These findings can have implications in further understanding the role of altered GSL expression in various pathological conditions and could be a target for future therapies.

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

Reference288 articles.

1. Varki, A., Cummings, R.D., and Esko, J.D. (2022). Essentials of Glycobiology, Cold Spring Harbor Laboratory Press. [4th ed.]. Chapter 11.

2. Structure and Function of Sphingoglycolipids in Transmembrane Signalling and Cell-Cell Interactions;Hakomori;Biochem. Soc. Trans.,1993

3. Functional Role of Glycosphingolipids in Cell Recognition and Signaling;Hakomori;J. Biochem.,1995

4. Cell Adhesion, Spreading, and Motility of G(M3)-Expressing Cells Based on Glycolipid-Glycolipid Interaction;Kojima;J. Biol. Chem.,1991

5. Structure and Function of Glycosphingolipids and Sphingolipids: Recollections and Future Trends;Hakomori;Biochim. Biophys. Acta-Gen. Subj.,2008

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. MALDI TIMS IMS Reveals Ganglioside Molecular Diversity within Murine S. aureus Kidney Tissue Abscesses;Journal of the American Society for Mass Spectrometry;2024-07-25

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3