Surface Properties of a Biocompatible Thermoplastic Polyurethane and Its Anti-Adhesive Effect against E. coli and S. aureus

Author:

Restivo Elisa12ORCID,Peluso Emanuela12ORCID,Bloise Nora123ORCID,Bello Giovanni Lo4,Bruni Giovanna5ORCID,Giannaccari Marialaura12ORCID,Raiteri Roberto4ORCID,Fassina Lorenzo6ORCID,Visai Livia123ORCID

Affiliation:

1. Department of Molecular Medicine, Centre for Health Technologies (CHT), Consorzio Interuniversitario Nazionale per la Scienza e la Tecnologia dei Materiali (INSTM), Research Unit (UdR) Pavia, University of Pavia, 27100 Pavia, Italy

2. Interuniversity Center for the Promotion of the 3Rs Principles in Teaching and Research (Centro 3R), University of Pavia Unit, 27100 Pavia, Italy

3. Medicina Clinica-Specialistica, UOR5 Laboratorio di Nanotecnologie, ICS Maugeri, IRCCS, 27100 Pavia, Italy

4. Department of Informatics, Bioengineering, Robotics and System Engineering—DIBRIS, University of Genoa, 16145 Genoa, Italy

5. Department of Chemistry, Physical Chemistry Section, University of Pavia, 27100 Pavia, Italy

6. Department of Electrical, Computer and Biomedical Engineering, Centre for Health Technologies (CHT), University of Pavia, 27100 Pavia, Italy

Abstract

Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on E. coli, while the bar coater significantly reduced staphylococcal planktonic adhesion and both bacterial biofilms compared to other samples. Interestingly, Pearson’s R coefficient analysis showed that Ra roughness and Haralick’s correlation feature were trend predictors for planktonic bacterial cells adhesion. The surface adhesion property was evaluated against NIH-3T3 murine fibroblasts by MTT and against human fibrinogen and human platelet-rich plasma by ELISA and LDH assay, respectively. An indirect cytotoxicity experiment against NIH-3T3 confirmed the biocompatibility of the TPUs. Overall, the results indicated that the deposition techniques did not alter the antibacterial and anti-adhesive surface properties of modified TPU compared to un-modified TPU, nor its bio- and hemocompatibility, confirming the suitability of TPU brush and bar coater films in the biomedical and pharmaceutical fields.

Publisher

MDPI AG

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