Occurrence of Multidrug-Resistant Bacteria Resulting from the Selective Pressure of Antibiotics: A Comprehensive Analysis of ESBL K. pneumoniae and MRSP Isolated in a Dog with Rhinorrhea
-
Published:2023-05-02
Issue:5
Volume:10
Page:326
-
ISSN:2306-7381
-
Container-title:Veterinary Sciences
-
language:en
-
Short-container-title:Veterinary Sciences
Author:
Rodrigues Inês C.12ORCID, Ribeiro-Almeida Marisa13, Ribeiro Jorge1ORCID, Silveira Leonor4, Prata Joana C.15ORCID, Pista Angela4ORCID, Martins da Costa Paulo12ORCID
Affiliation:
1. School of Medicine and Biomedical Sciences, University of Porto (ICBAS-UP), Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal 2. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), Terminal de Cruzeiros do Porto, de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal 3. UCIBIO—Applied Molecular Biosciences Unit, Rede de Química e Tecnologia (REQUIMTE), Laboratory of Microbiology, Department of Biological Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal 4. Department of Infectious Diseases, National Institute of Health, Av. Padre Cruz, 1600-609 Lisbon, Portugal 5. TOXRUN—Toxicology Research Unit, University Institute of Health Sciences, Cooperativa de Ensino Superior Politécnico e Universitário (CESPU), 4585-116 Gandra, Portugal
Abstract
Because of public health concerns, much greater scrutiny is now placed on antibiotic use in pets, especially for antimicrobial agents that have human analogs. Therefore, this study aimed to characterize the phenotypic and genotypic profiles of multidrug-resistant bacteria isolated from nasal swabs samples taken from a one-year-old male Serra da Estrela dog with rhinorrhea that was treated with amikacin. An extended-spectrum β-lactamases (ESBL) Klebsiella pneumoniae was isolated in the first sample taken from the left nasal cavity of the dog. Seven days later, methicillin-resistant (MRSP) Staphylococcus pseudintermedius was also isolated. Nevertheless, no alterations to the therapeutic protocol were performed. Once the inhibitory action of the antibiotic disappeared, the competitive advantage of the amikacin-resistant MRSP was lost, and only commensal flora was observed on both nasal cavities. The genotypic profile of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae revealed the same characteristics and close relation to other strains, mainly from Estonia, Slovakia and Romania. Regarding MRSP isolates, although resistance to aminoglycosides was present in the first MRSP, the second isolate carried aac(6′)-aph(2″), which enhanced its resistance to amikacin. However, the veterinary action was focused on the treatment of the primary agent (ESBL K. pneumoniae), and the antibiotic applied was according to its phenotypic profile, which may have led to the resolution of the infectious process. Therefore, this study highlights the importance of targeted therapy, proper clinical practice and laboratory-hospital communication to safeguard animal, human and environmental health.
Subject
General Veterinary
Reference58 articles.
1. Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis;Murray;Lancet,2022 2. Palma, E., Tilocca, B., and Roncada, P. (2020). Antimicrobial resistance in veterinary medicine: An overview. Int. J. Mol. Sci., 21. 3. Occurrence of multidrug resistant Gram-negative bacteria and resistance genes in semi-aquatic wildlife—Trachemys scripta, Neovison vison and Lutra lutra—As sentinels of environmental health;Mengistu;Sci. Total Environ.,2022 4. Grakh, K., Mittal, D., Kumar, T., Thakur, S., Panwar, D., Singh, L., Kumar, M., and Jindal, N. (2022). Attitude, opinions, and working preferences survey among pet practitioners relating to antimicrobials in India. Antibiotics, 11. 5. Skalet, A.H., Cevallos, V., Ayele, B., Gebre, T., Zhou, Z., Jorgensen, J.H., Zerihun, M., Habte, D., Assefa, Y., and Emerson, P.M. (2010). Antibiotic selection pressure and macrolide resistance in nasopharyngeal Streptococcus pneumoniae: A cluster-randomized clinical trial. PLoS Med., 7.
|
|