Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China-Reference-Cited by-同舟云学术

Genetic and In Vitro Characteristics of a Porcine Circovirus Type 3 Isolate from Northeast China

Published:2023-08-10 Issue:8 Volume:10 Page:517
ISSN:2306-7381
Container-title:Veterinary Sciences
language:en
Short-container-title:Veterinary Sciences

Author:

Wang Menghang¹,Yu Ying²,Wu Jianan¹,Wang Shujie¹^ORCID,Giménez-Lirola Luis G³^ORCID,Piñeyro Pablo³^ORCID,Wang Yu¹,Cui Hongliang¹,He Xijun¹,Zimmerman Jeffrey J.³^ORCID,Tu Yabin¹,Cai Xuehui¹,Wang Gang¹⁴

Affiliation:

1. Heilongjiang Research Center for Veterinary Biopharmaceutical Technology, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

2. College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China

3. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA

4. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agricultural University, Tai’an 271002, China

Abstract

Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding the pathogenicity of PCV3, mostly due to the limited number of PCV3 isolates that are readily available. In this study, PCV3-DB-1 was isolated in PK-15 cells and characterized in vitro. Electron microscopy revealed the presence of PCV-like particles, and in situ hybridization RNA analysis demonstrated the replication of PCV3 in PK-15 cell culture. Based on phylogenetic analysis of PCV3 isolates from the Heilongjiang province of China, PCV3-DB-1 with 24 alanine and 27 lysine in the Cap protein was originally isolated and determined to belong to the clade PCV3a.

Funder

State Key Laboratory of Veterinary Biotechnology Foundation

Central Public-interest Scientific Institution Basal Research Fund

Ph.D. foundation of Qingdao Agricultural University, China

Publisher

MDPI AG

Subject

General Veterinary

Link

https://www.mdpi.com/2306-7381/10/8/517/pdf

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