Affiliation:
1. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China
2. Xianghu Laboratory, Hangzhou 311231, China
3. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310032, China
Abstract
Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs), a biologically active compound derived from selenium polysaccharides, have demonstrated potential in addressing obesity. However, the mechanism through which LCS-SeNPs alleviate high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) remains unclear. Our results elucidated that LCS-SeNPs significantly inhibited fat accumulation and markedly improved the intestinal barrier by increasing mucus secretion from goblet cells. Moreover, LCS-SeNPs reshaped intestinal flora composition by increasing the abundance of mucus-associated microbiota (Bifidobacterium, Akkermansia, and Muribaculaceae_unclassified) and decreasing the abundance of obesity-contributed bacterium (Anaerotruncus, Lachnoclostridium, and Proteus). The modulation of intestinal microbiota by LCS-SeNPs influenced several metabolic pathways, including bile acid secretion, purine metabolites, and tryptophan derivation. Meanwhile, glycocholic acid and tauro-beta-muricholic acid were significantly reduced in the LCS-SeNP group. Our study suggests the crucial role of intestinal microbiota composition and metabolism, providing a new theoretical foundation for utilizing selenium polysaccharides in the intervention of HFD-induced NAFLD.
Funder
Department of Science and Technology of Zhejiang Province
Key Laboratory for Efficient Development and Utilization of Deep Sea Fisheries Resources of Zhejiang Province
Ministry of Science and Technology of the People’s Republic of China
Bureau of Science and Technology of Zhoushan
Xianghu Laboratory
Department of Science and Technology of Shandong Province