Affiliation:
1. Center for Natural and Human Sciences, Federal University of ABC, Santo André 09210-580, SP, Brazil
Abstract
The first element legislated adopting chemical speciation was chromium (Cr) for differentiation between the highly toxic Cr(VI) from the micronutrient Cr(III). Therefore, this work aimed to develop a new analytical method through the coupling of High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) with inductively coupled plasma mass spectrometry (ICP-MS) to obtain molecular and elemental information simultaneously from a single sample injection. In the first step, a low-cost flow split made of acrylic was developed aiming at optimally directing the sample to the detectors, enabling the HPLC-DAD/ICP-MS coupling. After the extraction of Certified Reference Materials (CRM of natural water NIST1640a and sugar cane leaf agro FC_012017), the recoveries determined by ICP-MS were 99.7% and 85.4%, respectively. Then, the method of HPLC-DAD/ICP-MS was applied for real samples of the CRMs. The presence of possible biomolecules associated with Cr(III) and Cr(VI) species was evaluated, with the simultaneous response detection of molecular (DAD) and elementary (ICP-MS) detectors. Potential biomolecules were observed during the monitoring of Cr(VI) and Cr(III) in sugar cane leaves, water samples and a supplement of Cr picolinate. Finally, the article also discusses the potential of the technique applied to biomolecules containing other associated elements and the need of more bioanalytical methods to understand the presence of trace elements in biomolecules.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health
Reference38 articles.
1. CETESB—Companhia Ambiental do Estado de São Paulo (2019). Qualidade das Águas Subterrâneas No Estado de São Paulo 2016–2018, CETESB—Companhia Ambiental do Estado de São Paulo.
2. Chromium and its speciation in water samples by HPLC/ICP-MS—Technique estabilishing metrological traceability: A review since 2000;Markiewicz;Talanta,2015
3. Chromium genotoxicity: A double-edge sword;Nickens;Chem. Biol. Interact.,2010
4. CONAMA-Conselho Nacional do Meio Ambiente (CONAMA) from the Brazilian Ministry of the Environment (2023, March 07). Resolução CONAMA nº 357 (DOU nº 053): Classificação dos Corpos de Água e Condições e Padrões de Lançamento de Efluentes, Available online: conama.mma.gov.br/?option=com_sisconama&task=arquivo.download&id=450.
5. A novel strategy for Cr(III) and Cr(VI) analysis in dietary supplements by speciated isotope dilution mass spectrometry;Unceta;Talanta,2016