Abstract
Multidrug-resistant bacteria present resistance mechanisms against β-lactam antibiotics, such as Extended-Spectrum Beta-lactamases (ESBL) and Metallo-β-lactamases enzymes (MBLs) which are operon encoded in Gram-negative species. Likewise, Gram-positive bacteria have evolved other mechanisms through mec genes, which encode modified penicillin-binding proteins (PBP2). This study aimed to determine the presence and spread of β-lactam antibiotic resistance genes and the microbiome circulating in Quito’s Public Transport (QTP). A total of 29 station turnstiles were swabbed to extract the surface environmental DNA. PCRs were performed to detect the presence of 13 antibiotic resistance genes and to identify and to amplify 16S rDNA for barcoding, followed by clone analysis, Sanger sequencing, and BLAST search. ESBL genes blaTEM-1 and blaCTX-M-1 and MBL genes blaOXA-181 and mecA were detected along QPT stations, blaTEM being the most widely spread. Two subvariants were found for blaTEM-1, blaCTX-M-1, and blaOXA-181. Almost half of the circulating bacteria found at QPT stations were common human microbiota species, including those classified by the WHO as pathogens of critical and high-priority surveillance. β-lactam antibiotic resistance genes are prevalent throughout QPT. This is the first report of blaOXA-181 in environmental samples in Ecuador. Moreover, we detected a new putative variant of this gene. Some commensal coagulase-negative bacteria may have a role as mecA resistance reservoirs.
Funder
Corporación Ecuatoriana para el Desarrollo de la Investigación y Academia
Universidad de las Américas
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health
Reference57 articles.
1. Catalano, A., Iacopetta, D., Ceramella, J., Scumaci, D., Giuzio, F., Saturnino, C., Aquaro, S., Rosano, C., and Sinicropi, M.S. (2022). Multidrug Resistance (MDR): A Widespread Phenomenon in Pharmacological Therapies. Molecules, 27.
2. Towards implementing an antibiotic stewardship programme (ASP) in Ecuador: Evaluating antibiotic consumption and the impact of an ASP in a tertiary hospital according to World Health Organization (WHO) recommendations;J. Glob. Antimicrob. Resist.,2021
3. Origins of the β-lactam rings in natural products;Tahlan;J. Antibiot.,2013
4. β-Lactamases and β-Lactamase Inhibitors in the 21st Century;Tooke;J. Mol. Biol.,2019
5. Extended-spectrum β-lactamases: An update on their characteristics, epidemiology and detection;Castanheira;JAC-Antimicrob. Resist.,2021
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