First Detection and Genetic Characterization of Swine Orthopneumovirus from Domestic Pig Farms in the Republic of Korea

Author:

Park Jonghyun123,Kim Hye-Ryung23,Lee Eon-Bee13ORCID,Lee Sang-Kwon3,Kim Won-Il4ORCID,Lyoo Young S.5,Park Choi-Kyu3,Ku Bok Kyung6ORCID,Jeoung Hye-Young6,Lee Kyoung-Ki6,Park Seung-Chun13ORCID

Affiliation:

1. Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, Institute for Animal Biomedical Science, College of Veterinary Medicine, Kyungpook National University, 80 Daehak-ro, Daegu 41566, Republic of Korea

2. DIVA Bio Incorporation, Daegu 41519, Republic of Korea

3. Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea

4. College of Veterinary Medicine, Jeonbuk National University, 79 Gobong-ro, Iksan 54596, Republic of Korea

5. College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea

6. Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea

Abstract

Novel swine orthopneumovirus (SOV) infections have been identified in pigs in the USA and some European countries but not in Asian countries, including South Korea, to date. The current study reports the first SOV infections in four domestic pig farms located in four provinces across South Korea. The detection rate of SOV in oral fluid samples using qRT-PCR was 4.4% (14/389), indicating the presence of the virus in pigs at commercial farms in Korea. Two complete genome sequences and one glycoprotein (G) gene sequence were obtained from SOV-positive samples. The complete genome analysis of KSOV-2201 and KSOV-2202 strains showed 98.2 and 95.4% homologies with a previously reported SOV, and the phylogenetic tree exhibited a high correlation with a previously reported SOV strain from the US and a canine pneumovirus (CPnV) strain from China. Based on the genetic analysis of the viral G gene, the murine pneumonia virus (MPV)-like orthopneumoviruses (MLOVs) were divided into two genogroups (G1 and G2). Seventeen CPnVs and two feline pneumoviruses were grouped into G1, while the Korean SOV strains identified in this study were grouped into G2 along with one SOV and two CPnVs. These results will contribute to expanding our understanding of the geographical distribution and genetic characteristics of the novel SOV in the global pig population.

Funder

Animal and Plant Quarantine Agency (APQA), the Ministry of Agriculture, Food and Rural Affairs, and the Republic of Korea

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

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