A Single-Cell Electroporation Model for Quantitatively Estimating the Pore Area Ratio by High-Frequency Irreversible Electroporation

Author:

Ding Lujia12,Fang Zheng12,Moser Michael A. J.3,Zhang Wenjun1ORCID,Zhang Bing2ORCID

Affiliation:

1. Division of Biomedical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK S7N 5A9, Canada

2. Intelligent Energy-Based Tumor Ablation Laboratory, School of Mechatronic Engineering and Automation, Shanghai University, Shanghai 200444, China

3. Department of Surgery, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada

Abstract

The electroporation technique utilizes pulsed electric fields to induce porous defects in the cell membrane, and the technique can be used for delivering drugs into cells and killing cancer cells. To develop an electric pulse protocol in the clinic with this technique, the key issue is to understand the evolution of pores in the cell membrane during the process of electroporation. This paper presents a study to address this issue. Specifically, a mathematical model of single-cell electroporation (SCE) was developed, which includes pore area ratio (PAR) as an indicator of the electroporation dynamics and area weight for considering the 3D nature of cells. The model was employed to simulate the electroporation of a single cell with different high-frequency irreversible electroporation (H-FIRE) protocols. The simulation result has found that the change of PAR with respect to the time duration of electroporation follows a sigmoid pattern to increase under specific protocols, which is called the cumulative effect of PAR. Subsequently, the relationship between the protocol of H-FIRE, described by a set of pulse parameters such as pulse width, pulse delay, electric field strength, and pulse burst duration, and the cumulative effect of PAR was established, which thereby allows designing the protocol to kill cells effectively. The study concluded that the proposed SCE model, along with the cumulative effect of PAR, is useful in designing H-FIRE protocols for the ablation of cancer tumors in the clinic.

Funder

National Natural Science Foundation of China

Chinese Scholarship Council

NSERC (Natural Sciences and Engineering Research Council of Canada) CREATE

Publisher

MDPI AG

Subject

Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science

Reference64 articles.

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