Affiliation:
1. School of Agriculture, Ludong University, Yantai 264025, China
2. Department of Plant Science, University of Cambridge, Cambridge CB2 3EA, UK
3. Yantai Growth Drivers Conversion Research Institute and Yantai Science and Technology Achievement Transfer and Transformation Demonstration Base, Yantai 264001, China
Abstract
Oyster mushroom spherical virus (OMSV) is a mycovirus that inhibits mycelial growth, induces malformation symptoms, and decreases the yield of fruiting bodies in Pleurotus ostreatus. However, the pathogenic mechanism of OMSV infection in P. ostreatus is poorly understood. In this study, RNA sequencing (RNA-seq) was conducted, identifying 354 differentially expressed genes (DEGs) in the mycelium of P. ostreatus during OMSV infection. Verifying the RNA-seq data through quantitative real-time polymerase chain reaction on 15 DEGs confirmed the consistency of gene expression trends. Both Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses highlighted the pivotal role of primary metabolic pathways in OMSV infection. Additionally, significant changes were noted in the gene expression levels of carbohydrate-active enzymes (CAZymes), which are crucial for providing the carbohydrates needed for fungal growth, development, and reproduction by degrading renewable lignocellulose. The activities of carboxymethyl cellulase, laccase, and amylase decreased, whereas chitinase activity increased, suggesting a potential mechanism by which OMSV influenced mycelial growth through modulating CAZyme activities. Therefore, this study provided insights into the pathogenic mechanisms triggered by OMSV in P. ostreatus.
Funder
Yantai Science and Technology Development Project, Shandong Province, China
Youth Innovation Technology Support Planning Project for Institution of Higher Education of Shandong Province
National Natural Science Foundation of China
Edible Fruiting bodies Genetic Breeding Innovation Team of Shandong Agricultural Industry Technology System