Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation-Reference-Cited by-同舟云学术

Effect of Sodium Thiosulfate Pre-Treatment on Renal Ischemia-Reperfusion Injury in Kidney Transplantation

Published:2024-09-02 Issue:17 Volume:25 Page:9529
ISSN:1422-0067
Container-title:International Journal of Molecular Sciences
language:en
Short-container-title:IJMS

Author:

Nelson Pierce¹²^ORCID,Dugbartey George J.²³⁴⁵^ORCID,McFarlane Liam¹²,McLeod Patrick²^ORCID,Major Sally²,Jiang Jifu²,O’Neil Caroline⁶^ORCID,Haig Aaron⁷,Sener Alp¹²³⁴

Affiliation:

1. Department of Microbiology & Immunology, Schulich School of Medicine and Dentistry, Western University, London, ON N6A 5C1, Canada

2. Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, Western University, London, ON N6A 5A5, Canada

3. Multi-Organ Transplant Program, London Health Sciences Center, Western University, London, ON N6A 5A5, Canada

4. London Health Sciences Center, Department of Surgery, Western University, London, ON N6A 5A5, Canada

5. Department of Pharmacology & Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Accra P.O. Box LG43, Ghana

6. The Molecular Pathology Core, Robarts Research Institute, London, ON N6A 5A5, Canada

7. Department of Pathology and Laboratory Medicine, Western University, London, ON N6A 5A5, Canada

Abstract

We recently reported in a rat model of kidney transplantation that the addition of sodium thiosulfate (STS) to organ preservation solution improved renal graft quality and prolonged recipient survival. The present study investigates whether STS pre-treatment would produce a similar effect. In vitro, rat kidney epithelial cells were treated with 150 μM STS before and/or during exposure to hypoxia followed by reoxygenation. In vivo, donor rats were treated with PBS or 2.4 mg/kg STS 30 min before donor kidneys were procured and stored in UW or UW+150 μM STS solution at 4 °C for 24 h. Renal grafts were then transplanted into bilaterally nephrectomised recipient rats which were then sacrificed on post-operative day 3. STS pre-treatment significantly reduced cell death compared to untreated and other treated cells in vitro (p < 0.05), which corresponded with our in vivo result (p < 0.05). However, no significant differences were observed in other parameters of tissue injury. Our results suggest that STS pre-treatment may improve renal graft function after transplantation.

Funder

Department of Surgery Internal Research Fund at Western University

a Canada Graduate Scholarship from the Canadian Institutes of Health Research

Publisher

MDPI AG

Link

https://www.mdpi.com/1422-0067/25/17/9529/pdf

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