Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study
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Published:2024-09-06
Issue:17
Volume:25
Page:9641
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Ksovreli Mariam1, Kachlishvili Tinatin1, Skhvitaridze Mariam1, Nadaraia Lili2, Goliadze Rusudan1, Kamashidze Luka1, Zurabiani Knarita1, Batsatsashvili Tatuli1, Kvachantiradze Nino1, Gverdtsiteli Marekhi3, Kantaria Temur3, Piot Olivier4ORCID, Courageot Marie-Pierre4, Terryn Christine5ORCID, Tchelidze Pavel6, Katsarava Ramaz3ORCID, Kulikova Nina1ORCID
Affiliation:
1. Institute of Cellular and Molecular Biology, Agricultural University of Georgia, 0159 Tbilisi, Georgia 2. Carl Zeiss Scientific and Education Center, New Vision University, 0159 Tbilisi, Georgia 3. Institute of Chemistry and Molecular Engineering, Agricultural University of Georgia, 0159 Tbilisi, Georgia 4. BioSpectroscopie Translationnelle (BioSpecT) Unit, University of Reims Champagne-Ardenne, 51100 Reims, France 5. La plateforme en Imagerie Cellulaire et Tissulaire (PICT), University of Reims Champagne-Ardenne, 51100 Reims, France 6. Faculty of Healthcare, East European University, 0159 Tbilisi, Georgia
Abstract
Our research explores leucine-based pseudo-proteins (LPPs) for advanced wound dressings, focusing on their effects on wound healing in an in vitro model. We assessed three types of LPP films for their ability to enhance wound closure rates and modulate cytokine production. They all significantly improved wound closure compared to traditional methods, with the 8L6 and copolymer films showing the most pronounced effects. Notably, the latter exhibited an optimal cytokine profile: an initial burst of pro-inflammatory TNF-α, followed by a controlled release of IL-6 during the proliferative phase and a significant increase in anti-inflammatory IL-10 during remodeling. This balanced cytokine response suggests that the copolymer film not only accelerates wound closure but also supports a well-regulated healing process, potentially reducing fibrosis and abnormal scarring, underscoring the potential of copolymer LPPs as advanced wound dressing materials. Future research will aim to elucidate the specific signaling pathways activated by the copolymer LPP to better understand its mechanism of action. Overall, LPP films offer a promising approach to improving wound care and could lead to more effective treatments for complex wounds.
Funder
Shota Rustaveli National Research Foundation
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