Whole-Genome Sequencing of Two Canine Herpesvirus 1 (CaHV-1) Isolates and Clinicopathological Outcomes of Infection in French Bulldog Puppies-Reference-Cited by-同舟云学术

Whole-Genome Sequencing of Two Canine Herpesvirus 1 (CaHV-1) Isolates and Clinicopathological Outcomes of Infection in French Bulldog Puppies

Published:2024-01-30 Issue:2 Volume:16 Page:209
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Rocchigiani Angela Maria¹,Bertoldi Loris²^ORCID,Coradduzza Elisabetta¹^ORCID,Lostia Giada¹,Pintus Davide¹,Scivoli Rosario¹^ORCID,Cancedda Maria Giovanna¹,Fiori Mariangela Stefania¹^ORCID,Bechere Roberto¹,Murtino Anna Pina¹,Pala Giovanni¹,Cardeti Giusy³,Macioccu Simona¹,Dettori Maria Antonietta¹,Pintore Antonio¹,Ligios Ciriaco¹,Puggioni Giantonella¹^ORCID

Affiliation:

1. Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy

2. BMR Genomics s.r.l., 35131 Padova, Italy

3. Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy

Abstract

Canine herpesvirus 1 (CaHV-1) infects dogs, causing neonatal death and ocular, neurological, respiratory, and reproductive problems in adults. Although CaHV-1 is widespread in canine populations, only four studies have focused on the CaHV-1 whole genome. In such context, two CaHV-1 strains from both the kidney and spleen of 20-day-old deceased French Bulldog puppies were recently isolated in Sardinia, Italy. The extracted viral DNA underwent whole-genome sequencing using the Illumina MiSeq platform. The Italian CaHV-1 genomes were nearly identical (>99%), shared the same tree branch, and clustered near the ELAL-1 (MW353125) and BTU-1 (KX828242) strains, enlarging the completely separated clade discussed by Lewin et al., in 2020. This study aims to provide new insights on the evolution of the CaHV-1, based on high-resolution whole-genome phylogenetic analysis, and on its clinicopathological characterization during a fatal outbreak in puppies.

Funder

Experimental Zooprophylactic In-stitute of Sardinia

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4915/16/2/209/pdf

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