Tapered Fiber Bioprobe Based on U-Shaped Fiber Transmission for Immunoassay
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Published:2023-10-20
Issue:10
Volume:13
Page:940
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ISSN:2079-6374
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Container-title:Biosensors
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language:en
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Short-container-title:Biosensors
Author:
Chen Xinghong123ORCID, Xiao Lei124, Li Xuejin1235, Yi Duo123, Zhang Jinghan1235, Yuan Hao123, Ning Zhiyao123, Hong Xueming123, Chen Yuzhi123ORCID
Affiliation:
1. School of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China 2. Shenzhen Engineering Laboratory for Optical Fiber Sensors and Networks, Shenzhen 518060, China 3. Shenzhen Key Laboratory of Sensor Technology, Shenzhen 518060, China 4. Shenzhen Tian’an Zhiyuan Sensor Technology Co., Ltd., Shenzhen 518060, China 5. School of Science, The Chinese University of Hong Kong, Shenzhen 518172, China
Abstract
In this paper, a tapered fiber bioprobe based on Mach–Zehnder interference (MZI) is proposed. To retain the highly sensitive straight-tapered fiber MZI sensing structure, we designed a U-shaped transmission fiber structure for the collection of optical sensing signals to achieve a miniature-insert-probe design. The spectrum responses from the conventional straight-tapered fiber MZI sensor and our proposed sensor were compared and analyzed, and experimental results showed that our proposed sensor not only has the same sensing capability as the straight-tapered fiber sensor, but also has the advantages of being flexible, convenient, and less liquid-consuming, which are attributed to the inserted probe design. The tapered fiber bioprobe obtained a sensitivity of 1611.27 nm/RIU in the refractive index detection range of 1.3326–1.3414. Finally, immunoassays for different concentrations of human immunoglobulin G were achieved with the tapered fiber bioprobe through surface functionalization, and the detection limit was 45 ng/mL. Our tapered fiber bioprobe has the insert-probe advantages of simpleness, convenience, and fast operation. Simultaneously, it is low-cost, highly sensitive, and has a low detection limit, which means it has potential applications in immunoassays and early medical diagnosis.
Funder
National Natural Science Foundation of China Shenzhen Bay Laboratory Open Fund Project High-end Talent Scientific Research Startup Project Natural Science Foundation of Guangdong Science and Technology plan project of Shenzhen LingChuang Research Project of the China National Nuclear Corporation
Subject
Clinical Biochemistry,General Medicine,Analytical Chemistry,Biotechnology,Instrumentation,Biomedical Engineering,Engineering (miscellaneous)
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