Complete Mitogenome and Phylogenetic Analysis of a Marine Ray-Finned Fish, Alcichthys elongatus (Perciformes: Cottidae)
Author:
Patil Maheshkumar Prakash1ORCID, Kim Jong-Oh23ORCID, Yoo Seung Hyun3, Seo Yong Bae4, Lee Yu-Jin5, Kim Jin-Koo5, Kitamura Shin-Ichi6ORCID, Kim Gun-Do23ORCID
Affiliation:
1. Industry-University Cooperation Foundation, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea 2. Department of Microbiology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea 3. School of Marine and Fisheries Life Science, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea 4. Research Institute for Basic Science, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea 5. Department of Marine Biology, Pukyong National University, 45 Yongso-ro, Nam-Gu, Busan 48513, Republic of Korea 6. Center for Marine Environmental Studies, 3 Bunkyo-cho, Matsuyama 790-8577, Japan
Abstract
Alcichthys elongatus is the only species in the genus, and this work is the first to provide a comprehensive mitogenome analysis of this species. The A. elongatus mitogenome was 16,712 bp long, with biased A + T content (52.33%), and featured thirteen protein-coding genes (PCGs), twenty-two tRNAs, two rRNAs, and the control region (D-loop). The H strand encoded twenty-eight genes (twelve PCGs, fourteen tRNA, and two rRNA) and the control region, whereas the L strand encoded the remaining nine genes (ND6 and eight tRNA). Except for COXI, which started with GTG, all PCG sequences started with ATG and ended with TAA (ND4L, ND5, COXI, ATP8) or TAG (ND1, ND6) termination codons, with some (ND2, ND3, ND4, COXII, COXIII, ATP6, Cytb) having an incomplete termination codon. Except for tRNA-serine-1 (trnS), the majority of the tRNAs exhibited characteristic cloverleaf secondary structures. Based on 13 PCGs, phylogenetic analysis placed A. elongatus in the same clade as Icelus spatula. This genomic data will be useful for species identification, phylogenetic analysis, and population genetics.
Funder
Ministry of Oceans and Fisheries, Korea
Subject
Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics
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