The Effect of Dietary Lipid Supplementation on the Serum Biochemistry, Antioxidant Responses, Initial Immunity, and mTOR Pathway of Juvenile Tilapia (Oreochromis niloticus)
Author:
Liu Yongqiang123ORCID, Huang Enhao123, Xie Yi123, Meng Liuqing123, Liu Dongsheng123, Zhang Ziqi123, Zhou Jiang123, Zhang Qin123, Tong Tong123
Affiliation:
1. Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning 530008, China 2. Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Guangxi Minzu University, Nanning 530008, China 3. School of Marine Sciences and Biotechnology, Guangxi Minzu University, 158 University Road, Nanning 530008, China
Abstract
The objectives of this study were to investigate the effect of different dietary lipid levels on the serum biochemistry, antioxidant responses, initial immunity, and mTOR pathway of juvenile genetic improvement-farmed tilapia (GIFT, Oreochromis niloticus). Six groups of the juveniles (initial body weight 20.66 ± 1.33 g) in triplicate were fed for 90 days using six iso-nitrogen diets with different lipid levels (0.35%, 3.35%, 6.35%, 9.35%, 12.35%, and 15.35%). The main results were as follows: Compared with the control group (0.35%), the diets with different lipid supplementation significantly improved (p < 0.05) the contents of total protein (TP), albumin (ALB), globulin (GLB), glucose (GLU), triglyceride (TG), total cholesterol (T-CHO), high-density lipoprotein (HDL), low-density lipoprotein (LDL), total antioxidant capacity (T-AOC), malondialdehyde (MDA), complement 3 (C3), and immunoglobulin M (IgM), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), lysozyme (LYZ), and alkaline phosphatase (ALP), the expression level of phosphatidylinositol-3-kinase (PI3K), Akt protein kinase B (Akt), and mammalian target of rapamycin (mTOR) genes in juvenile tilapia. However, diets with different lipid supplementation significantly reduced (p < 0.05) the expression level of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) genes of juvenile tilapia. In conclusion, the with different lipid supplementation could significantly affect the serum biochemistry, antioxidant responses, initial immunity, and mTOR pathway of juvenile tilapia.
Funder
grants from the Scientific Research Foundation for the Introduced Talents of Guangxi Minzu University the Research Capacity Enhancement Project for Young and Middle-aged Teachers in Guangxi Universities the Joint Funds of the National Natural Science Foundation of China the Shandong Provincial Key Research and Development Programs the Innovation-driven Development Special Fund Project of Guangxi the Grants from the Scientific Research Foundation for the Introduced Talents of Guangxi Minzu University
Subject
Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics
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