Abstract
Na+/H+-exchanger (NHE) assumes a significant part in different particle transport in creatures. A clone of Penaeus monodon NHE cDNA was examined in this study (PmNHE), and its impact on high-concentration ammonia nitrogen stress was researched. The 877-amino acid (aa) protein was encoded by a full-length PmNHE cDNA that was 2788 base pairs (bp) long and had a 2643-bp open reading frame (ORF). The findings show that PmNHE was expressed in all of the P. monodon organs that were tested, including the intestine, muscle, hemolymph, heart, hepatopancreas, stomach, epidermis, gill, testis, and ovary, and the intestine and muscle were found to have the highest levels of PmNHE expression. The expression of PmNHE in the gill tissue of P. monodon was significantly up-regulated under high levels of ammonia nitrogen stress. The expression of PmNHE in the intestine of P. monodon under high-concentration ammonia nitrogen stress was significant. When exposed to high concentrations of ammonia nitrogen stress, P. monodon exhibited shorter survival times than the two control groups. Hence, it is suggested in the present study that PmNHE may have a significant impact on the environment with high levels of ammonia nitrogen.
Funder
National Key R&D Program of China
China Agriculture Research System
Hainan Yazhou Bay Seed Laboratory
Research and Development Projects in Key Areas of Guangdong Province
Central Public interest Scientific Institution Basal Research Fund, CAFS
Central Public Interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS
Hainan Provincial Natural Science Foundation of China
Guangdong Basic and Applied Basic Research Foundation
Guangzhou Science and Technology Planning Project
Hainan Provincial Association for Science and Technology of Young Science and Technology Talents Innovation Plan Project
Subject
Ocean Engineering,Water Science and Technology,Civil and Structural Engineering