Growth and Physiological Responses of Chrysotila roscoffensis (Coccolithales) to Different Phosphorus Resources

Abstract

The growth and physiological process of microalgae interact with environmental nutrients. In the present study, we compared the growth and physiological characteristics of coccolithophore Chrysotila roscoffensis under the effects of phosphate at high and low concentrations, and of organic phosphorus of adenosine triphosphate (ATP) and Na2-glycerophosphate (SG). The growth, photosynthesis, calcification, alkaline phosphatase activity, and inorganic nutrient absorption rate were comparatively studied. The results showed that the culture with a low concentration of initial phosphate (6 μM) had a lower growth rate than that with a high concentration (45 μM). However, a relatively similar maximum cell density could be achieved. Equimolar inorganic phosphorus supported a higher initial growth rate than organic phosphorus. ATP was better than SG at supporting higher growth rates, higher photosynthetic activity, and higher cell density. Cellular alkaline phosphatase (AP) responded rapidly to nutrient variations with sharp changes of activities, independent of the initial P resources. Cellular calcification was at a higher level in groups with lower growth rates. Phosphate in low concentration in the medium was not absorbed during the early growth period while that in high concentration was rapidly absorbed. Instead, phosphate in low concentration was rapidly absorbed in the late stationary phase. The absorption of nitrate was affected by the initial P resources as well. The DIN/DIP ratio in the water varied significantly during the growth periods. The results indicated that C. roscoffensis had flexible physiological strategies in utilizing varied phosphorus resources, and high cell density maintenance of C. roscoffensis may play roles in nutrient conditions in the water. This study may help to extend the understanding of nutrient utilization strategy in microalgae and to apply reference in the application of Chrysotila species in the removal of nutrient pollution.