Anti-Human Immunodeficiency Virus-1 Property of Thai Herbal Extract Kerra™-Reference-Cited by-同舟云学术

Anti-Human Immunodeficiency Virus-1 Property of Thai Herbal Extract Kerra™

Published:2024-07-09 Issue:7 Volume:17 Page:917
ISSN:1424-8247
Container-title:Pharmaceuticals
language:en
Short-container-title:Pharmaceuticals

Author:

Saehlee Siriwan¹,Seetaha Supaphorn¹,Klankaew Wiwat²,Srathong Pussadee³^ORCID,Choowongkomon Kiattawee¹²^ORCID,Choengpanya Khuanjarat⁴^ORCID

Affiliation:

1. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand

2. Interdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand

3. Faculty of Nursing, Praboromarajchanok Institute, Nonthaburi 11000, Thailand

4. Program in Basic Science, Maejo University–Phrae Campus, Phrae 54140, Thailand

Abstract

Kerra™, a Thai traditional herbal medicine derived from the “Tak-Ka-Si-La Scripture” and composed of nine medicinal plants, has demonstrated potential antiviral properties against HIV. This study investigated the inhibitory effects of Kerra™ on HIV-1 reverse transcriptase (RT) and its ability to prevent pseudo-HIV viral infection in HEK293 cells. The results showed that Kerra™ extract achieved a 95.73 ± 4.24% relative inhibition of HIV-1 RT, with an IC50 value of 42.66 ± 8.74 µg/mL. Docking studies revealed that key phytochemicals in Kerra™, such as oleamide, formononetin, and biochanin A, interact with several residues in the RT non-nucleoside binding pocket, contributing to their inhibitory effects. Furthermore, Kerra™ was able to reduce pseudo-HIV infection in HEK293 cells at a concentration of 10 µg/mL, suggesting its potential as a supplementary treatment for HIV.

Funder

Kasetsart University Research and Development Institute

Publisher

MDPI AG

Link

https://www.mdpi.com/1424-8247/17/7/917/pdf

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