Affiliation:
1. Council for Agricultural Research and Economics, Research Centre for Agriculture and Environment, Via di Corticella 133, 40128 Bologna, Italy
2. Council for Agricultural Research and Economics, Research Centre for Animal Production and Aquaculture, Via Beccastecca 345, 41018 San Cesario s/P, Italy
Abstract
This study aimed to evaluate the microbial oil production by three selected strains, Mortierella isabellina, Cunninghamella echinulata, and Thamnidium elegans, after fermentation of an enzymatic hydrolysate from alkali pre-treated giant reed biomass, in comparison to a semi-synthetic medium, at three increasing nitrogen supplementation levels (0.14, 0.25, and 0.47 g/L). M. isabellina showed the fastest sugar consumption, the highest final cell and oil concentrations (10.9 and 5.6 g/L, respectively), as well as the highest cellular oil content, oil yield/g sugar consumed, and oil productivity (63.4%, 0.19 g/g, and 1 g/L/day, respectively) in the giant reed hydrolysate. The oil yield tended to decrease with an increasing nitrogen content in the cultures. Oleic acid was the most copious fatty acid in the oil for all the fungi. On the contrary, T. elegans exhibited the poorest performances. In particular, M. isabellina showed, respectively, the highest and lowest values of oleic and γ-linolenic acid (52.2 and 3.1%, on average). In comparison, C. echinulata and T. elegans showed much higher γ-linolenic acid content (15.3 and 21.6%, on average). Notably, the C. echinulata cultures showed by far the highest γ-linolenic acid concentration in both substrates (345 and 595 g/L in the giant reed hydrolysate and in the synthetic medium, respectively). Finally, the estimated biodiesel properties of all the oils fell within the limits of the U.S. standards, while the oil of M. isabellina only respected the tighter limits fixed by the E.U. regulations.
Funder
Ministry of Agricultural, Food and Forestry Policies
Subject
Energy (miscellaneous),Energy Engineering and Power Technology,Renewable Energy, Sustainability and the Environment,Electrical and Electronic Engineering,Control and Optimization,Engineering (miscellaneous),Building and Construction
Reference75 articles.
1. Third Generation Biofuels from Microalgae;Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology,2010
2. Mota, M., Múgica, P., and Sá-Correia, I. (2022). Exploring Yeast Diversity to Produce Lipid-Based Biofuels from Agro-Forestry and Industrial Organic Residues. J. Fungi, 8.
3. Microbial Oil Production from Rice Straw Hydrolysate by Trichosporon fermentans;Huang;Bioresour. Technol.,2009
4. Biomass and Methane Yield of Giant Reed (Arundo donax L.) as Affected by Single and Double Annual Harvest;Ceotto;GCB Bioenergy,2021
5. Valdes, G., Mendonca, R.T., and Aggelis, G. (2020). Lignocellulosic Biomass as a Substrate for Oleaginous Microorganisms: A Review. Appl. Sci., 10.