Abstract
It has been hypothesized that α-Klotho deficiency might contribute to chronic inflammation in patients with end-stage renal disease (ESRD), especially those on hemodialysis (HD). Serum Klotho levels by some authors are considered a potential predictor of cerebrovascular events. Therefore, we analyzed serum levels of α-Klotho with ELISA and inflammation-related cytokines in HD patients. Sixty-seven HD patients and 19 healthy people were recruited between November 2017 and June 2021. A Cytometric Bead Array (CBA) was used to determine the level of different cytokines: IL-12p70, TNF, IL-10, IL-6, IL-1β, and IL-8. A human Klotho ELISA kit was used to determine the level of α-Klotho in the plasma samples of HD patients. There was no difference in serum levels of α-Klotho between HD patients and healthy people. Patients had increased serum IL-6 and IL-8. Significant positive correlations existed between the concentration of α-Klotho and the serum concentrations of IL-12p70, IL-10, and IL-1β. However, in a multivariable linear regression analysis, only patients’ age was associated independently with α-Klotho level. Serum α-Klotho was not associated with higher mortality risk in HD patients. While these results draw attention to potential relationships between α-Klotho proteins and inflammatory markers in HD patients, our cross-sectional study could not confirm the pathogenic link between α-Klotho, inflammation, and cardiovascular mortality.
Funder
the statutory funds of the Medical University of Gdańsk
the Excellence Initiative research
Reference47 articles.
1. Mutation of the mouse klotho gene leads to a syndrome resembling ageing;Kuro-O;Nature,1997
2. Klotho and aging;Kuro-O;Biochim. Biophys. Acta,2009
3. Regulation of fibroblast growth factor-23 signaling by klotho;Kurosu;J. Biol. Chem.,2006
4. Tissue expression and source of circulating αKlotho;Olauson;Bone,2017
5. Identification of the human klotho gene and its two transcripts encoding membrane and secreted klotho protein;Matsumuraab;Biochem. Biophys. Res. Commun.,1998
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