Acetaldehyde Stimulation of the Growth of Zymomonas mobilis Subjected to Ethanol and Other Environmental Stresses: Effect of Other Metabolic Electron Acceptors and Evidence for a Mechanism

Abstract

Ethanol-stressed cultures of Z. mobilis showed greatly reduced lag times in growth when supplemented with small amounts of acetaldehyde. This effect could be mimicked by other metabolic electron acceptors, including propionaldehyde and oxygen, indicating a redox-based mechanism. Added propionaldehyde was rapidly and stoichiometrically converted to 1-propanol, suggesting that added acetaldehyde is also reduced during early growth. Acetaldehyde addition measurably accelerated glycolysis in nongrowing cells and also slightly stimulated cultures subjected to temperature change, osmotic shock and salt and acetate stress. Acetaldehyde’s stimulatory effect appears to be due to its ability to accelerate glycolysis via its effect on the cellular redox balance. Acetaldehyde reduction opposes the drain on NAD+ concentrations caused by oxidation of the added ethanol, accounting for the particularly strong effect on ethanol-stressed cells. This study provides evidence for our earlier proposed redox-based mechanism for acetaldehyde’s ability to reduce the lag phase of environmentally stressed cultures and suggests that the effect may have applications in industrial fermentations, especially those inhibited by ethanol and toxic compounds present in, for instance, lignocellulosic hydrolysates.