Performance and Bacterial Characteristics of Aerobic Granular Sludge in Treatment of Ultra-Hypersaline Mustard Tuber Wastewater

Abstract

Mustard tuber wastewater (MTW) is an ultra-hypersaline high-strength acid organic wastewater. Aerobic granular sludge (AGS) has been demonstrated to have high tolerance to high organic loading rate (OLR), high salinity, and broad pH ranges. However, most studies were conducted under single stress, and the performance of AGS under multiple stresses (high salinity, high OLR, and low pH) was still unclear. Herein, mature AGS was used to try to treat the real MTW at 9% salinity, pH of 4.1–6.7, and OLR of 1.8–7.2 kg COD/m3·d. The OLR was increased, and the results showed that the upper OLR boundary of AGS was 5.4 kg COD/m3·d (pH of 4.2) with relatively compact structure and high removal of TOC (~93.1%), NH4+-N (~88.2%), and TP (~50.6%). Under 7.2 kg COD/m3·d (pH of 4.1), most of the AGS was fragmented, primarily due to the multiple stresses. 16S rRNA sequencing indicated that Halomonas dominated the reactor during the whole process with the presence of unclassified-f-Flavobacteriaceae, Aequorivita, Paracoccus, Bradymonas, and Cryomorpha, which played key roles in the removal of TOC, nitrogen, and phosphorus. This study investigated the performance of AGS under multiple stresses, and also brought a new route for highly-efficient simultaneous nitrification–denitrifying phosphorus removal of real MTW.