Solid-State Fermentation as a Sustainable Tool for Extracting Phenolic Compounds from Cascalote Pods
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Published:2023-09-08
Issue:9
Volume:9
Page:823
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ISSN:2311-5637
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Container-title:Fermentation
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language:en
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Short-container-title:Fermentation
Author:
López-Cárdenas Francisco12, Ochoa-Reyes Emilio2ORCID, Baeza-Jiménez Ramiro2ORCID, Tafolla-Arellano Julio C.1ORCID, Ascacio-Valdés Juan A.3ORCID, Buenrostro-Figueroa José J.2ORCID
Affiliation:
1. Laboratorio de Biotecnología y Biología Molecular, Departamento de Ciencias Básicas, Universidad Autónoma Agraria Antonio Narro, Saltillo 25315, Mexico 2. Laboratory of Biotechnology & Bioengineering, Research Center in Food and Development A.C., Delicias 33089, Mexico 3. Bioprocess & Bioproducts Research Group, Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo 25280, Mexico
Abstract
Cascalote pods are an important source of phenolic compounds, mainly recovered using solvent extraction methods. To find a sustainable alternative to these methods, this study aimed to evaluate solid-state fermentation (SSF) in order to enhance the extractability of total phenolic compounds (TPC) with antioxidant activity (AA) from cascalote pods. Aspergillus niger GH1 was selected based on the amount of TPC released and AA measured (ABTS, DPPH, FRAP) in a short period of time. Moreover, moisture, temperature, inoculum size, and mineral composition were evaluated. The largest amount of TPC released was 124.17 mg/gdw (g of dry weight) after 12 h of culture, which significantly correlated with the AA (Pearson’s R = 0.94). Moisture and KH2PO4 concentration were the main influencing factors of TPC release. Treatment 6 (1 × 107 spores/gdw, 30 °C, 60% moisture, mineral composition (g/L): KH2PO4, 1.52; NaNO3, 7.65; and MgSO4, 1.52) was selected due to the highest values of both TPC and AA. SSF-assisted extraction allowed for an increase of 118% and 93% in TPC and AA values, respectively. Corilagin, lagerstannin, geraniin, and ellagic acid were the main phenolic compounds identified by RP-HPLC-ESI-MS in the cascalote extracts. The results obtained demonstrate the feasibility of SSF-assisted extraction as a biotechnological alternative for the recovery of important bioactive molecules from this underutilized material.
Subject
Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science
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