Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification

Abstract

Coproduction of multienzymes from single potential microbe has captivated contemplation in industries. Bacterial strain, Halomonas meridiana VITSVRP14, isolated from seaweed was labored to produce amylase, agarase and xylanase conjointly using submerged fermentation. The optimum production conditions clinched by classical optimization were: pH 8; 1.5% inoculum; 24 h incubation, 40 °C; 8% NaCl (sodium chloride); 1% lactose and NaNO3 (sodium nitrate). The preponderant variables (pH, temperature, lactose) and their interaction effect on enzyme production were studied by Plackett-Burman design and Response Surface Methodology (RSM). There were 3.29, 1.81 and 2.08 fold increase in enzyme activity with respect to agarase, amylase and xylanase after optimization against basal medium. After 24 h of enzymatic treatment, the saccharification rates of the coproduced enzyme mixture were 38.96% on rice bran, 49.85% on wheat bran, 61.2% on cassava bagasse and 57.82% on corn cob. Thus, the coproduced enzyme mixture from a bacterium with halotolerance is plausible in pretreated lignocellulose degradation. The ability of this single microbe Halomonas meridiana VITSVRP14, in coproducing agarase, amylase and xylanase give the nod for its application in biomass saccharification by subsiding cost, energy and time involved in the process.